Figure 7

Effect of SIRT1, SIRT2, and MOF depletion, and sirtuin inhibitors on DNA compaction during RAF-induced senescence of WI-38hTERT/GFP-RAF-ER. A) DNA 4',6'-diamidino-2-phenylindole (DAPI) staining of representative nuclei and corresponding DAPI coefficient of variation (CV) values (from Figure 6B). B) Boxplots of DAPI CV (n > 60, three experiments are shown). *DNA compaction statistically different from No Target (P < 10–5, Welch t-test). C) DNA DAPI staining of representative nuclei and corresponding DAPI CV values. D) Boxplots of DAPI CV (n > 80, from one experiment). *DNA compaction statistically different from solvent DMSO control (P < 10–5, Welch t-test). E) Immunoblot showing the level of H4-K16Ac in acid-extracted histones. Loading control: H3. DMSO: WI-38hTERT/GFP-RAF-ER + 0.1% dimethyl sulfoxide (DMSO) + 20 nM 4-HT (3 days); Salermide: WI-38hTERT/GFP-RAF-ER + 50 μM salermide + 20 nM 4-HT (3 days); Sirtinol: 25 μM sirtinol + 20 nM 4-HT (3 days).