Figure 3
Distribution of male-specific lethal (MSL) complex subunits on polytene chromosomes of larvae expressing a Flag-tagged maleless (MLE)(ΔRB2) protein. Indirect immunofluorescence was performed on polytene chromosomes from salivary glands of y w1118/w; pr mle1/pr mle1; H83msl2/hsp83-Flag-mle(ΔRB2)w+ females. (B-D) MSL1, MSL3 and H4K16ac were restricted to the high-affinity sites, whereas (A, E) MLE was associated with additional sites and was present prominently in the chromocenter (white arrows). Similar results (data not shown) were obtained on salivary-gland polytene chromosomes from females expressing the hsp83-Flag-mle(ΔRB1,2)w+ transgene. (F, G) Control females homozygous for (F) H83 msl2 and mle1(G) H83 msl2 and Flag-mle+. For the wild-type distribution of H4K16ac please see Figure 2H.