Figure 2
Distribution of male-specific lethal (MSL) complex subunits on polytene chromosomes of larvae expressing a Flag-tagged maleless (MLE)(ΔRB1) protein. (A-E) Indirect immunofluorescence was performed on polytene chromosomes from salivary glands of y w1118/w; pr mle1/pr mle1; H83msl2/hsp83-Flag-mle(ΔRB1)w+ females. The only MLE epitope present was the product of the transgene. Preparations were stained with the two antisera indicated, and yellow signals an overlap of the respective epitopes. (F, G) Distribution of the roX RNAs in the presence of Flag-tagged MLE(ΔRB1) protein. In situ hybridization was performed on salivary-gland polytene chromosomes from females of the genotype indicated above. (H) Polytene chromosomes of a w1118/w; pr mle1/pr mle1; H83msl2/hsp83-Flag-mle+w+ control female stained with anti-H4K16ac and anti-MSL1 sera. As expected, there was complete overlap of the two epitopes.