Figure 4

Target genes bound by both Myc and Miz-1 have a distinct, transcriptionally inactive profile of histone marks. (A) Examples of enrichment profiles observed for Myc, Miz-1, acetylated lysine 9 of histone H3 (AcH3K9) (AcK9) and trimethylated lysine 4 of histone H3 (H3K4me3) (TMK4) for Myc/Miz-1, Miz-1 alone or Myc alone bound genes are shown. Miz-1, Myc, TMK4, and AcK9 are indicated in blue, red, green, and purple, respectively. Black horizontal arrows indicate direction of transcription. Peaks are on a Log2 scale. Gene names are indicated above the colored horizontal bars with tiled regions immediately below and transcriptional start sites (TSSs) are indicated by short vertical lines near the bottom. (B) Chromatin immunoprecipitation (ChIP) analysis of H3K27me3 enrichment on Myc/Miz-1 and Myc alone bound genes. (C) Myc and Miz-1 binding distribution relative to the TSS.