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Figure 3 | Epigenetics & Chromatin

Figure 3

From: Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatin

Figure 3

Cell-cycle regulation of SMCX (Smcy homolog, X-linked (mouse)). (A) SMCX levels in cells treated with and stained for (5-bromo-2'-deoxyuridine, BrdU). Cells were exposed to BrdU for 3 hours then double-stained with anti-BrdU and SMCX antibodies. Representative cells (high or low BrdU staining) are shown. Top two rows show cells exhibiting the granular pattern. (B) SMCX DNA was transfected into cells, and 2 days after transfection cells were synchronized with aphidicolin. One day later, cells were released from the aphidicolin block, and SMCX levels were monitored at indicated intervals using western blotting. (C) This experiment was designed as in (B), except that transfection was not performed and endogenous SMCX levels were monitored. Cyclin A = cyclin A levels, a cell-cycle synchronization control; cyclin A levels increase in late G1 and stay high through the S phase. H3 = histone H3, a loading control for the chromatin fraction. Other terminology as in Figure 5.

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Epigenetics & Chromatin

ISSN: 1756-8935