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Figure 1 | Epigenetics & Chromatin

Figure 1

From: Pluripotency factor binding and Tsix expression act synergistically to repress Xist in undifferentiated embryonic stem cells

Figure 1

Analysis of Nanog binding to Xist Intron 1 in ES cells carrying a targeted inversion in Xist. (A) Schematic representation of the wt and INV Xist alleles. Arrows indicate the position of the NOS binding site. (B) RNA FISH images showing Xist (green) and Tsix (red) expression in wt ES cells (129/1) and those carrying a targeted inversion in the Xist locus (NBXT INV1). Arrowheads point to punctate signal. DNA was counterstained with DAPI (blue). (C) Occupancy of the Oct4 promoter and Xist intron 1 by Nanog in wt ES cells (129/1) and NBXT INV1. XEN cells which do not express Nanog were used as a negative control. The promoter of the housekeeping gene B2M was used as a negative control for Nanog binding. DAPI: 4',6-diamidino-2-phenylindole; ES: embryonic stem; FISH: fluorescent in situ hybridisation; NBXT INV1: Xist allele carrying a targeted inversion; wt: wild type; XEN: extraembryonic endoderm.

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Epigenetics & Chromatin

ISSN: 1756-8935