Figure 5

High-resolution analysis of centromere protein (CENP)-A and histone H3 dimethylated at lysine 4 (H3K4me2) at promoters in the vicinity of neocentromeres. Area of (a) ~4 kb at the promoter region of the RAP2A gene near the neocentromere in IMS13q (see Figure 3a); (b) ~4.8 kb at the promoter of the TPP2 gene in BBB (see Figure 2); (c) ~1500 bp at the c13orf gene in BBB; (d) ~4 kb at the KdelC1 and BIVM genes in BBB. (e) ~2200 bp at the ERCC5 gene in BBB. For each panel (a-e), the neocentric CENP-A domain is shown, and the H3K4me2 domains for both BBB and IMS13q lines. The transcription start site and CpG island for each gene are indicated. In general, the CENP-A is found in a distinct location from the H3K4me2 and appears to favor CpG islands.