Figure 2
From: Repressive and non-repressive chromatin at native telomeres in Saccharomyces cerevisiae
The Sir complex is required for the formation of repressive chromatin at a native telomere. (A) Frequency of fluoroorotic acid (FOA) resistance in isogenic Δsir::KanMX strains containing the URA3-yEGFP marker adjacent to the core X element at the indicated telomere. The mean and standard deviation of FOA resistance is given for each strain. (B) Chromatin structures of the XIL and IIIR telomeres were analysed in Δsir1::KanMX, Δsir2::KanMX, Δsir3::KanMX and Δsir4::KanMX strains, by MNase digestion and indirect end labelling, as described for Figure 1B.