Suppression of heterochromatic silencing in trans heterozygotes of RNA Pol II 140 and RNA silencing machinery mutants. The genotypes of control, single heterozygous and trans-heterozygous male flies are indicated involving the X chromosome inversion In(1)w [m4h]. The effect on DX1 tandem mini-white silencing is also shown in the middle. The genotypes of control, single heterozygous and trans-heterozygous male flies are indicated. The alellic combinations used are noted. Measurement of eye pigment analysis is presented below. Measurements from three independent experiments are shown. Error bars represents standard error. Optical density values at 480 nm are indicated on the Y-axis and the genotypes represented on the X-axis. Asterisks indicate significant P values < 0.05.