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Figure 2 | Epigenetics & Chromatin

Figure 2

From: Epigenetic inheritance of an inducibly nucleosome-depleted promoter and its associated transcriptional state in the apparent absence of transcriptional activators

Figure 2

The naked PHO5 promoter can be inherited through DNA replication even in the absence of the activators. (a) Schematic of experimental outline, showing flow cytometry DNA content profiles for the strains JKT0010 (WT) and JMY0002 (spt6-140) at the indicated temperatures. '-Pi A' refers to asynchronously growing in phosphate-free media, while the remainder of the -Pi samples are at various stages of the cell cycle, as indicated by the flow cytometry analyses below. (b) Inactivation of Spt6 prevents repression of PHO5 even following DNA replication. Acid phosphatase activity of the PHO5 gene product was measured at the indicated times in the schematic shown in (a). (c) Schematic of locations of primer pairs used for the chromatin immunoprecipitation (ChIP) analyses shown below. (d) ChIP analysis of H3 occupancy at the PHO5 TATA region. (e) Quantitation of ChIP analysis of H3 occupancy at the PHO5 TATA region, normalized to the TELVIR internal control. (f) ChIP analysis of H3 occupancy at the PHO5 UAS region. (g) Quantitation of ChIP analysis of H3 occupancy at the PHO5 UAS region, normalized to the TELVIR internal control. (h) ChIP analysis of H3 occupancy at the PHO5 adjacent region. (i) Quantitation of ChIP analysis of H3 occupancy at the PHO5 adjacent region, normalized to the TELVIR internal control.

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Epigenetics & Chromatin

ISSN: 1756-8935