Figure 4

Expression profiling shows that white is selectively down-regulated in w^m4e. A-C) MA plots of expression profiles of log₂ Oregon-R-S Self-self (A), log₂ (w^m4e/Oregon-R-S) (B), and log₂ (w^m51b/Oregon-R-S) (C), respectively. In these plots, for each gene, change in expression (log₂) is plotted against the expression level. A = average fluorescence intensity (log₂ √(Cy5xCy3)). Dashed lines indicate four times the standard deviation. D) qRT-PCR measurements of changes in gene expression in w^mmutants versus wild-type (Oregon-R-S). Bars show average log₂ ratios of five replicate experiments. Error bars represent standard deviations. Expression levels were normalized to the housekeeping gene Ide, which is located on chr 3L; arm is located outside of X_Syx4-CG3603 and serves as an additional control.