Expression profiling shows that white is selectively down-regulated in wm4e. A-C) MA plots of expression profiles of log2 Oregon-R-S Self-self (A), log2 (wm4e/Oregon-R-S) (B), and log2 (wm51b/Oregon-R-S) (C), respectively. In these plots, for each gene, change in expression (log2) is plotted against the expression level. A = average fluorescence intensity (log2 √(Cy5xCy3)). Dashed lines indicate four times the standard deviation. D) qRT-PCR measurements of changes in gene expression in wmmutants versus wild-type (Oregon-R-S). Bars show average log2 ratios of five replicate experiments. Error bars represent standard deviations. Expression levels were normalized to the housekeeping gene Ide, which is located on chr 3L; arm is located outside of XSyx4-CG3603 and serves as an additional control.