Anti-5meC immunostaining of rabbit one-cell nuclear transfer embryos. Rabbit one-cell nuclear transfer (NT) embryos were fixed 4 hours after activation. Immunostaining with α-5meC antibody shows the absence of signal in the majority (A, n = 12) of NT embryos, while a partial (B, n = 3) or complete (C, n = 4) presence of signal is observed less frequently. (D) Control staining on rabbit fetal fibroblasts used as donors for NT. Scale bar 20 μm.