Figure 8
Expression of genes near telomeres is unaffected by nst mutations. Total RNA was extracted [47] from N3120 (nst+) and N3132 (nst-1RIP 1nst-3RIP 1nst-5RIP 1) and 20 μg was separated by agarose gel, blotted, and probed as described [48]. The 28S rRNA panel shows equivalent loading in both lanes by methylene blue staining of a blot. Other panels are autoradiograms after probing with the coding region of the gene closest to telomere IL (NCU010129.3), VIL (NCU07143.3), or VIIL (NCU09306.3).