Figure 7

Analyses of DNA methylation near telomeres. DNA was digested with isoschizomers DpnII (D) and Sau3AI (S), separated on agarose gels, blotted, and probed with telomere proximal regions to assay DNA methylation in these regions. Strains for the TelVR hph panel: N2292, the primary transformant with hph targeted to tel VR (T); N3120, a nst⁺ strain (WT); N3004, an hpo strain (hpo); N3132, the triple nst-1, -3, -5 mutant (nst). The hph probe was the first 600 bp of the coding region. Strains for the tel VIIL bar panel: N3440, the primary transformant with bar targeted to tel VIIL (T); N3441 (nst⁺); N3443 (hpo). The bar probe was the entire coding region. For the other panels, DNA samples of a wildtype strain, N150 (WT), an hpo mutant, N3004 (hpo), and N3132, a triple nst-1 nst-3 nst-5 mutant (nst) were processed as for the other panels and probed for: telomere IL (Tel IL; 226–1249 bp from end); telomere IVL (TelIVL; 2500–3657 bp from end); telomere VR (Tel VR; 1130–2887 bp from end); telomere VIIL (Tel VIIL; 324–1306 bp from end); a methylated repeat called 8:F10 (see text).