Figure 6

Analysis of de novo DNA methyltransferases in Dicer-deficient XY embryonic stem cell lines. Western blot analysis of Dnmt1 (A), Dnmt3b (B) and Dnmt3a (C) in Pgk12.1 XX (Pgk), 129/1 (129), Dicer^lox/lox (F/F) and Dicer-deficient (Di Δ/Δ) XY embryonic stem (ES) cell lines. Lamin B was used as a loading control. Quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis of Dnmt1, Dnmt3b, Dnmt3a2 and Dnmt3L transcription in Dicer^lox/lox (Di F/F) and Dicer-deficient XY ES cell lines (D)-(F). Two or three primer pairs were used for each Dnmt and average data from triplicate measurements is shown. All data is normalised to Idh2 and β-actin transcript levels and presented relative to D3Cre Dnmt level for S5, S6 and E5 clones and to DTCM23 F/F for DTCM23 and DTCM49 groups of clones.