Volume 6 Supplement 1

Epigenetics and Chromatin: Interactions and processes

Open Access

XACT, a long non-coding transcript coating the active X chromosome in human pluripotent cells

  • Céline Vallot1, 2,
  • Christophe Huret2,
  • Yann Lesecque3,
  • Alissa Resch4,
  • Noufϊssa Oudrhiri5,
  • Annelise Bennaceur5,
  • Laurent Duret3 and
  • Claire Rougeulle1, 2
Epigenetics & Chromatin20136(Suppl 1):O33

DOI: 10.1186/1756-8935-6-S1-O33

Published: 18 March 2013

X-chromosome inactivation (XCI), the dosage compensation process that equalizes X-linked gene expression between sexes, has mostly been studied in the mouse, where the central role for the non-coding RNA Xist in the initiation and spreading of the process was demonstrated. Although Xist is conserved in humans [1], very little is known concerning its regulation and function in this species. Several lines of evidence moreover suggest that different strategies have been adopted in the human to control XCI as compared to the mouse. In particular, in human pre-implantation development, XIST RNA coats the X chromosome(s) in both male and female embryos without inducing X-chromosome silencing [2]. This indicates that XIST expression and X-inactivation can be uncoupled during human embryogenesis and that other elements likely participate to the control of X chromosome activity in humans.

XCI is established early during embryonic development, and embryonic stem cells can be used to decipher the kinetics and the molecular actors of the process. Human female embryonic stem cells (hESC) can be found in different configurations regarding XIST expression: most female hESC have already undergone XCI but tend to spontaneously lose XIST expression [3]. In the course of an RNA-seq analysis of female hESC, we identified an extended and un-annotated transcribed region producing a long unspliced, likely non-coding nuclear RNA. RNA-FISH analysis reveals that this transcript is expressed from, and coats the active X chromosome. We called this transcript XACT, for X-active coating transcript. In female hESC in which XIST is repressed, XACT is expressed from and coats both Xs, and this correlates with significant reactivation of the inactive X chromosome. Expression of XACT appears to be specific for pluripotent cells as its expression decreases during differentiation. Finally, we provide evidence that XACT is not conserved in the mouse.

In conclusion, we have identified XACT as the first long ncRNA that coats the active X chromosome in human. Given its expression profile and lack of conservation, it is tempting to speculate that XACT is involved in the peculiar control of XCI initiation in human.

Authors’ Affiliations

(1)
Univ Paris Diderot, Sorbonne Paris Cité, Epigenetics and Cell Fate
(2)
CNRS, UMR7216 Epigenetics and Cell Fate
(3)
Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université de Lyon, Université Lyon 1
(4)
Stem Cell Institute, UCHC
(5)
INSERM U935, Université Paris Sud 11, AP-HP

References

  1. Brown CJ, Lafreniere RG, Powers VE, Sebastio G, Ballabio A, Pettigrew AL, Ledbetter DH, Levy E, Craig IW, Willard HF: Localization of the X inactivation centre on the human X chromosome in Xq13. Nature. 1991, 34: 82-84.View ArticleGoogle Scholar
  2. Okamoto I, Patrat C, Thepot D, Peynot N, Faugue P, Daniel N, Diabangouaya P, Wolf JP, Renard JP, Duranthon V, Heard E: Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development. Nature. 2011, 472: 370-374. 10.1038/nature09872.View ArticlePubMedGoogle Scholar
  3. Makhlouf M, Rougeulle C: Linking X chromosome inactivation to pluripotency: Necessity or Fate?. Trend Mol Med. 2011, 17: 326-336.View ArticleGoogle Scholar

Copyright

© Vallot et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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