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Fig. 7 | Epigenetics & Chromatin

Fig. 7

From: The MMP-2 histone H3 N-terminal tail protease is selectively targeted to the transcription start sites of active genes

Fig. 7

CTSB is the secondary H3NT protease in U2OS cells a Venn diagram comparing all known and predicted proteases that were upregulated during U2OS cell expansion (left) to those that were downregulated in U2OS + 2 MMP-2 depleted cells (right). MMP-2 and CTSB are highlighted in the overlapping list (middle). b In vitro H3 cleavage assay using core histone substrates incubated alone or with recombinant MMP-2 (rMMP-2) positive control, recombinant CTSB (rCTSB), or nuclear soluble extracts purified from U2OS + 2 cells or C2C12 differentiated myotubes (MT). Western analysis was performed to detect the H3NT cleaved products observed in U2OS + 2 chromatin (red). c Western analysis of purified chromatin from 293 T negative control cells, C2C12 differentiated myotubes (MT), U2OS pLKO.1 subconfluent (− 2) and over-confluent (+ 2) cells and U2OS + 2 cells expressing pLKO.1-CTSB shRNAs (sh1 and sh2). The MMP-2 generated H3∆18 product and two CTSB-generated H3NT cleaved products are indicated (red). Amido black stain (AB) of the membrane shows equivalent loading of chromatin between samples

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