Fig. 3

MMP-2 displays sharp chromatin binding at 5’ transcription regulatory regions a Western analysis of nuclear extracts purified from 293 T negative control cells, C2C12 myoblasts (MB) and differentiated myotubes (MT), and U2OS + 2 wild type (WT) and ProMMP2-3xHA cells using an MMP-2 (top) or HA antibody (bottom). ProMMP2-3xHA and the endogenous MMP-2 pro-form and catalytically active form are indicated. b U2OS + 2 ProMMP2-3xHA cells were fractioned into soluble cytoplasmic (Cyto) and nuclear (NE) extracts. The remaining insoluble chromatin (Chr) was washed repeatedly with 600 mM NaCl and solubilized via sonication prior to SDS-PAGE. Western analysis was performed with an HA antibody. c Heatmap and average plot profile of merged ProMMP2-3xHA and wild type (WT) negative control HA-nChIP-Seq replicates. RPKM normalized signal is plotted for each, centered over the 7216 ProMMP2-3xHA called peaks and extending ± 4 kb. d The genome wide distribution of the 7216 ProMMP2-3xHA peaks at defined genomic elements (y-axis) was determined using log2(peak enrichment/random background) values from the Homer annotatePeaks package (x-axis)