Fig. 6

Global loss of 5mC and 5hmC in AKG-treated diabetic rats. A Representative images of immunohistochemical staining with 5mC antibody in cryosections of control and diabetic rats with or without AKG treatment and below is shown 5mC fluorescence intensity (Green) normalized to DAPI staining (Blue). B 5mC- and 5hmC-specific dot blot analysis of gDNA isolated from control and diabetic rats with or without AKG treatment. Equal loading of gDNA was assessed by methylene blue staining. Densitometry analysis of dot blots is shown with dot intensities normalized to methylene blue staining. The results are expressed as the mean ± SD in three different biological replicates. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons post-test, where *p < 0.05, **p < 0.001, ****p < 0.0001 vs control group, ##p < 0.001 ###p < 0.0002, ####p < 0.0001 vs STZ