Fig. 3

Validation of MEDIP/hMEDIP-seq data and functional relevance of 5mC/5hmC to gene expression. A Gene-specific enrichment of 5mC at DUDP26 and ATPAF2 in control and diabetic rats. B Gene-specific enrichment of 5hmC in the OGDH and PLN in control and diabetic rats. (n = 3). C, D qPCR analysis of relative mRNA levels in LV tissue of control and diabetic rat C For genes enriched in MEDIP-seq analysis, D For genes enriched in hMEDIP-seq analysis (n = 5) primers sequences are given in Additional file 1: table S5. For methylation and hydroxymethylation analysis, the relative enrichment of 5mC and 5hmC, respectively, was normalized to 10% input, and mRNA expression was normalized to the β-actin gene. The results are expressed as the mean ± SD of replicate values. Statistical significance was determined by Student’s unpaired t-test where *p < 0.05, **p < 0.001, ***p < 0.0002, ****p < 0.0001 with respect to control