Fig. 1
From: Caf1 regulates the histone methyltransferase activity of Ash1 by sensing unmodified histone H3
Caf1 binds to the proximal region of the Ash1 histone binding module cluster. A A series of the truncation constructs of Ash1 and their binding to Caf1. Strong binding is indicated with ‘+++’, weak binding with ‘+’ and no binding with ‘−’ (left panel) based on GST-pulldown experiments with GST-Ash1 constructs and Caf1 (right panel). GST-Ash1 constructs are marked in green dots and Caf1 with red dots. B Further truncation of Ash1_C1 and their binding to Caf1. The minimal region of Caf1 binding site on Ash1 is termed as ‘Caf1 Binding Motif (CBM)’. C The sequence conservation of CBM in Ash1s among Drosophila melanogaster, Homo sapiens, Mus musculus and Danio rerio. Absolutely conserved residues are highlighted in red, and partially conserved residues in yellow