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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Ovulatory signal-triggered chromatin remodeling in ovarian granulosa cells by HDAC2 phosphorylation activation-mediated histone deacetylation

Fig. 6

CK2α Nuclear Translocation is Required for HDAC2 Phosphorylation, H3K27Ac Deacetylation, and Ovulation. A Western blotting of CK2α in nuclear and cytoplasm fragmentation of ovaries at the indicated timepoints of PMSG or hCG. B A diagram depicting the experimental design. TBB is an inhibitor of CK2α. H0–H4: 0, 1, and 4 h after the hCG treatment. C Western blotting analysis for H3K27Ac, HDAC2, pHDAC2, ERK1/2, and pERK1/2 at different timepoints (0, 1, and 4 h) post-hCG in the control and TBB groups. D, E Immunofluorescence of pHDAC2 (D, red) and H3K27Ac (E, red) at different timepoints (0, 1, and 4 h) after hCG treatment in the control and TBB groups. Nuclei were co-stained with DAPI (n = 3). Scale bar = 100 μm. F Hematoxylin and eosin staining showed that the TTB treatment inhibits cumulus expansion and ovulation. The mice ovaries were collected at 14-h post-hCG in mice of the control and TBB groups (n = 9). Scale bar = 100 um. G The bar graph depicting the ovulated oocyte numbers from mice in the control and TBB groups (n = 9). Data are presented by the mean ± SD. P value was determined by t-test. ***P < 0.001. H RT-qPCR analysis of the mRNA levels of ovulatory specific genes, including Ereg, Star, and Sult1e1. The fold change was represented by setting the relative level of 0 h in the control group as 1. The results are represented as mean ± SD. *P < 0.05, **P < 0.01, calculated by one-way ANOVA followed by Turkey’s multiple comparisons tests

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