Fig. 2

The Ovulatory Hormone Signal Induces Genome-wide H3K27Ac Deacetylation and Subsequent Histone Acetylation. A A diagram exhibiting the sample collection for H3K27Ac ChIP-seq. H0, H1, H4: 0 h, 1 h and 4 h after hCG treatment. B H3K27Ac enrichment around peak center for ovaries at 0 h, 1 h, and 4 h post-hCG treatment. The upper panels show the average signal profile around detected peak centers (± 2 kb). The lower heatmap shows H3K27Ac read density for a total of 9,868 shared peaks of H0 and H4 around the peak centers. Two independent ChIP-seq experiments were sequenced, as were two matched input controls. C A Venn diagram of H3K27Ac ChIP-seq genes. H3K27Ac ChIP-seq was performed using the ovaries at 0 h, 1 h, and 4 h post-hCG treatment. The numbers indicate the total genes identified in each timepoint. D, E The box blot represents the average RPKM (reads per kilobase per million) of common H3K27Ac-enriched genes (D) and H3K27Ac-gain genes (E). The data are presented as the mean ± SD. F, G The UCSC Genome Browser tracks demonstrating the occupancy of H3K27Ac on Fshr and Lhcgr genes (representing the common H3K27Ac-enriched genes before and after ovulatory signal trigger) and Ereg, Sult1e1, and Star genes (belonging to the H3K27Ac-gain genes). H, I The ChIP-qPCR validation of common H3K27Ac-enriched genes and H3K27Ac-gain at 0, 1, and 4 h post-hCG injection. J The stacked bar chart showing the genomic distribution of H3K27Ac occupancy of common H3K27Ac-enriched genes and H3K27Ac-gain genes. K Venn diagram depicting the overlapped gene number of significantly upregulated genes after hCG induction (extracted from GSE119508) with H3K27Ac-gain or common H3K27Ac-enriched genes. L Gene Ontology (GO) analysis demonstrates the biological process (BP) of upregulated genes with H3K27Ac-gain post-hCG induction