Fig. 6

CDK4 is involved in MAZ-regulated pRCC cell proliferation. A RT-qPCR was used to examine CDK4 mRNA expression in different cell lines. B RT-qPCR was used to measure CDK4 mRNA levels in clinical tissues. C The Kaplan–Meier analysis was used to determine survival rates for patients with pRCC based on CDK4 levels. D The CDK4 promoter contains the potential binding site for MAZ. E ChIP-qPCR detected MAZ binding to CDK4 promoter region in Caki-2 cells. F Luciferase reporter assays were performed to detect CDK4 promoter activity in Caki-2 cells after cotransfected with indicated vectors. G ACHN cells were transfected with oeMAZ or shCDk4 or corresponding control vectors alone or together, and then cell viability was detected using MTT. H Caki-2 cells were transfected with shMAZ or shCDk4 or pLKO control vectors alone or together, and MTT was used to measure cell viability. I, J ACHN cells were transfected as in G, and colony formation assays was used to explore cell proliferation. All data are from three independent experiments and are expressed as mean ± standard error. *p < 0.05, **p < 0.01, ***p < 0.001 versus the corresponding controls