Fig. 4

MAZ is involved in PCGF6-regulated pRCC cells proliferation. A ACHN cells were transfected with MAZ overexpression vector (oeMAZ) or PCGF6 shRNA or corresponding control vectors alone or together, and then Western blotting was used to examine the protein levels of MAZ and CDK4. B Quantitative analysis of Western blotting from A. C Caki-2 cells were transfected with shMAZ or shPCGF6 or pLKO control vector alone or together, then MAZ and CDK4 protein level were detected by Western blot. D Quantitative analysis of Western blotting from C. E ACHN cells were transfected as in A, and cell viability was detected using MTT. F Caki-2 cells were transfected as in C, and MTT was used to measure cell viability. G, H ACHN cells were transfected as in A, and colony formation assays was used to explore cell proliferation. All data are from three independent experiments and are expressed as mean ± standard error. *p < 0.05, **p < 0.01 versus the corresponding controls