Skip to main content
Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: PCGF6/MAX/KDM5D facilitates MAZ/CDK4 axis expression and pRCC progression by hypomethylation of the DNA promoter

Fig. 3

MAZ is downstream molecular of PCGF6 in pRCC and is positively correlated with PCGF6 expression. A Caki-2 cells were transfected with two vectors of PCGF6 shRNAs (shPCGF6-1# and shPCGF6-2#), ACHN cells were transfected with PCGF6 overexpression vectors. Western blot analysis was used to examine MAZ protein expression. B Quantitative analysis of Western blotting from A. C The indicated vectors were transfected into cells as A, and the expression of MAZ was measured using RT-qPCR. D The mRNA level of MAZ was measured using RT-qPCR in tumor (n = 31) and healthy kidney (n = 31) tissues. E The protein expression of MAZ was measured using Western blotting in pRCC tissues. F Quantitative analysis of the data from Western blotting of E. G TCGA database was used to analyze mRNA expression of MAZ. H Kaplan–Meier analysis of the relationship between the expression level of MAZ and patient prognosis in the TCGA database. I Correlation analysis was performed between MAZ and PCGF6 mRNA expression in pRCC tissues. All data are from three independent experiments and are expressed as mean ± standard error. *p < 0.05, **p < 0.01, ***p < 0.001 versus the corresponding controls

Back to article page