Fig. 2

PCGF6 facilitates pRCC cell growth in vitro. A Caki-2 cells were transfected with two vectors of PCGF6 shRNAs (shPCGF6-1# and shPCGF6-2#), ACHN cells were transfected with PCGF6 overexpression vectors. RT-qPCR was conducted to measure the mRNA level of PCGF6. B The indicated vectors were transfected into cells as A, and the expression of PCGF6 was measured using Western blotting. C Quantitative analysis of Western blotting from B. D–F Cell transfection as A, and cell viability was detected using MTT (D) and colony formation assays (E, F). All data are from three independent experiments and are expressed as mean ± standard error. *p < 0.05, **p < 0.01 versus the corresponding controls