Skip to main content
Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: The transgenic IG-DMR sequence of the mouse Dlk1-Dio3 domain acquired imprinted DNA methylation during the post-fertilization period

Fig. 5Fig. 5

Bisulfite sequencing analysis of mIG-DMR transgene. A Enlarged map of the mIG-DMR transgene. The region (gray horizontal bar) analyzed by qMethyl-PCR in Fig. 4 is shown above the enlarged map. The regions amplified by nested PCR for BS sequencing and primer sets used are shown beneath the enlarged map. B Genomic DNA from sperm germ cells (two biological replicates) and tail-tip somatic cells of non-Tg mouse was subjected to BS sequencing. Location of repeat sequence is shown by gray rectangle. The CpG motifs in the AccII/HpaII recognition sites are marked by horizontal lines. C, D Genomic DNAs from germ cells (sperm or oocyte), blastocyst, and tail-tip somatic cells of TgM (lines 74 and 157 in C and D, respectively) inheriting the transgene either paternally (upper panels) or maternally (lower) were subjected to BS sequencing. ID numbers shown above each block correspond to those shown in Fig. 4B, C

Back to article page