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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: The transgenic IG-DMR sequence of the mouse Dlk1-Dio3 domain acquired imprinted DNA methylation during the post-fertilization period

Fig. 4

Methylation analysis of mIG-DMR transgene by qMethyl-PCR. A Structure of the mIG-DMR transgene. The region analyzed by qMethyl-PCR (B–D) is shown beneath the enlarged mIG-DMR map. The locations of DNA methylation-sensitive HpaII and AccII sites in the mIG-DMR, as well as the qMethyl-PCR target sequences, are shown by vertical lines. B, C Pedigree (non-transgenic individuals are not shown) and methylation level of the HpaII/AccII sites adjacent to the repeat sequence. The meaning of each object is same as those described in the legends to Fig. 2B, C. D Methylation levels of the paternally (p, blue) or maternally (m, red) inherited transgenes were compared and mean ± SD values for each line are graphically depicted. P values < 0.05 are considered significantly different

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