Fig. 1
From: Proximity labeling reveals a new in vivo network of interactors for the histone demethylase KDM5
TurboID-tagged KDM5 proteins are functional and biotinylate endogenous proteins. A Schematic of the workflow used for purifying and fingerprinting biotinylated proteins from adult heads using LC–MS/MS mass spectrometry. B Schematic of the four UASp constructs generated able to express HA-tagged TurboID alone, dCas9:TurboID, NT-KDM5 and CT-KDM5. C Western blot using adult heads showing levels of expression of KDM5 using anti-HA, biotinylation using a streptavidin-680 conjugate and the loading control alpha-tubulin. Genotypes: kdm5140; gkdm5:HA (a wild-type strain; Control), Ubi-Gal4/+ ; UASp-TurboID/+ (TurboID), Ubi-Gal4/+ ; UASp-dCas9:TurboID (dCas9:TurboID), kdm5140, Ubi-Gal4/kdm5140; UASp-NT- kdm5/+ (NT-KDM5) and kdm5140, Ubi-Gal4/kdm5140; UASp-CT- kdm5 /+ (CT-KDM5). D Rescue of kdm5140-induced lethality by ubiquitous expression of UAS-NT-kdm5 or UAS-CT-kdm5 using Ubi-Gal4. Genotype of male and female adult flies shown is kdm5140, Ubi-Gal4/kdm5140; UASp-NT- kdm5 /+ (NT-KDM5) and kdm5140, Ubi-Gal4/kdm5.140; UASp-CT- kdm5/+ (CT-KDM5)