Fig. 13

In vitro Pax6 binding to sites with a single unmethylated and methylated CpG dinucleotide. A The consensus motif 1–1 [86] is shown followed by individual binding sites with C residues methylated marked by asterisks (red). B EMSA results using Pax6 PD/HD and PD(5a)/HD proteins. The specificity of the complexes is demonstrated by competition with cold oligonucleotides containing consensus Pax6 binding sites (P6CON) and unlabeled self-oligonucleotide. The autoradiography of experiments with PD/HD and PD(5a)/HD proteins were 6 and 16 h, respectively