Fig. 2

Analysis of ATAC-seq data from embryo chicken left gonads. a PCA plot of ATAC-seq data from embryo chicken left gonads. b Correlation between DEGs and DARs in three pairwise comparisons. c Example of DEGs (DMRT1) showing gradually increasing expression pattern during female to male transition and their associated DARs, as well as DEGs (IGSF9B) showing gradually decreasing expression pattern during female to male transition and their associated DARs. ATAC-seq tracks are shown in the RPKM scale. The y axis of the RNA-seq boxplot shows their group name, and the x axis shows the mean rlog-normalized counts. d Venn diagrams show the shared and unique genes obtained from RNA-seq and ATAC-seq in three pairwise comparisons. e Top five enriched motifs in different biased peaks in three pairwise comparisons