Fig. 2
From: Phthalates impact on the epigenetic factors contributed specifically by the father at fertilization
Biological pathways enriched in response to dibutyl-phthalate (DBP). A DBP responsive enriched biological pathways were identified using the Molecular Signature Database (MSigDB). The total number of unique RE-RNAs and enriched gene sets is provided. Enriched gene sets were either unique to DBP study arm (B1HB2 or H1BH2) or within each study arm. B1HB2 provided the background DBP study arm baseline to crossover to crossback, H1BH2 provided the high-DBP study arm baseline to crossover to crossback. B DBP-responsive RNA Elements (REs) within the enriched biological pathways that share paternally provided RE-containing RNAs (RE-RNA) were identified. Paternally provided: REs determined as fivefold paternally enriched or twofold paternally enriched. Node color indicates the following; Orange: enriched biological processes, Yellow: RE abundance increases following DBP exposure, Pink: RE abundance decreases upon DBP exposure, Green: RE abundance increases upon DBP withdrawal, Blue: RE abundance decreases upon DBP withdrawal. Edge color indicates the total number of DBP responsive REs (1–40 REs) with an overlapping paternally provided RE-associated gene name moving between nodes. Scaling for edge color is continuous with 1 RE attributed to a Dark Blue color and 40 REs attributed to a Red color. Edge line type indicates the following; solid: background DBP (baseline visit) to high-DBP (crossover visit) (B1H), parallel lines: high-DBP (crossover visit) to background DBP (crossback visit) (HB2), dash: high-DBP (baseline visit) to background DBP (crossover visit) (H1B), dots: background DBP (crossover visit) to high-DBP (crossback visit) (BH2)