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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Efficient generation of epigenetic disease model mice by epigenome editing using the piggyBac transposon system

Fig. 3

Generation of epigenome-edited TG mice using the optimized PB system. a Vector-integrated (TG) embryos (18.5 dpc) were generated using optimized concentrations of hyPBase and PB vector containing H19-DMR gRNA. Non-TG:control mice in which the epigenome-editing vector did not successfully integrate. BF bright field. Scale bar, 1 cm. b Body and placental weights of TG and non-TG mice with H19-DMR gRNA or scrambled gRNA. **P < 0.01 (two-tailed Student’s t test). c Combined bisulfite restriction analysis (COBRA) for H19-DMR and promoter regions. Significant demethylation was observed in TG embryos with H19-DMR gRNA. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA followed by Tukey’s post hoc HSD test). d Amplicon bisulfite sequencing analysis targeting 59 CpG sites among three representative samples. TSS transcription start site

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