Fig. 2

Optimization of hyPBase and PB vector concentrations for generation of TG epigenome-edited mice. a Schematic of the generation of epigenome-edited mice by cytoplasmic injection of hyPBase and PB vector into fertilized eggs. b Embryos at the 2-cell stage (next day after injection). BF bright field. Scale bars, 250 μm. c Embryos at 11.5 dpc and the associated agarose gel electrophoresis image of PCR products targeting the dCas9 transgene. Red numbers indicate TG mice in which the PB vector is integrated. Scale bar, 5 mm. d TG efficiency at 11.5 dpc under a constant concentration ratio (1:7) of hyPBase to PB vector. e TG efficiency at 11.5 dpc under a constant PB vector concentration (7 ng/μL). *P < 0.05, **P < 0.01 (two-tailed Student’s t test)