Table 4 Sequences of primers used in plasmid construction and biotinylated DNA probe generation
Primer name | Primer sequence |
|---|---|
SJ-BYU-1 | AAGCTTATCCCATTCTCTCATCAATTGAGTGGGATGAGGCTATCTCTGCCTCTCTTCTGA |
SJ-BYU-2 | GCTAGCGTCATCCACAGTGTAATGTAAGATGGTTCAGAGATTCAGAAGAGAGGCAGAGAT |
CK-SJ-5 | ACTAGCAAGCTTGGAGATCCCTCGAAAATTTC |
CK-SJ-6 | GTGCTCAAGCTTGGAAATCTCCGGGGAATTTC |
CK-SJ-7 | ACTAGCGCTAGCGGAGATCCCTCGAAAATTTC |
CK-SJ-8 | GTGCTCGCTAGCGGAAATCTCCGGGGAATTTC |
HindIII601 F | AAGCTTACAGGATGTATATATCTGACACG |
HindIII601 R | AAGCTTACTGGAGAATCCCGGTCTGC |
NheI601 F | GCTAGCACAGGATGTATATATCTGACACG |
NheI601 R | GCTAGCACTGGAGAATCCCGGTCTGC |
NheI_5t | /5Phos/CTAGCTTTTTG |
NheI_5a | /5Phos/CTAGCAAAAAG |
HindIII_5t | /5Phos/AGCTTTTTTTA |
HindIII_5a | /5Phos/AGCTTAAAAAA |
N_R_polyT | /5Phos/CTAGCAAAAAAAAAAAAAAAAAAAAG |
N_F_polyT | /5Phos/CTAGCTTTTTTTTTTTTTTTTTTTTG |
H_R_polyT | /5Phos/AGCTTAAAAAAAAAAAAAAAAAAAAA |
H_F_polyT | /5Phos/AGCTTTTTTTTTTTTTTTTTTTTTTA |
70R_N_pSJ322 | GCTAGCAAATTATTGGTTTTTAATGTTTTTTGCTG |
70F_N_pSJ322 | GCTAGCTATTTCAAAAATAATTTTTCTAGTATAATTC |
70R_H_pSJ322 | AAGCTTAAATTATTGGTTTTTAATGTTTTTTGCTG |
70F_H_pSJ322 | AAGCTTTATTTCAAAAATAATTTTTCTAGTATAATTC |
0SMJ023 | /5Phos/CTAGCTTTTTGTTTTTG |
oSMJ024 | /5Phos/CTAGCAAAAACAAAAAG |
oSMJ118 | ATGGAAAAACGCCAGCAACG |
oSMJ119_5Biotin | TATGAGGACGGTATACATTCG |