Fig. 4

Liver function is impaired by chromatin decondensation and transcription activation. Gene ontology (GO) enrichment analysis of spheroids treated for A 1, B 2, C 3 days and D after recovery (day 10). Functional annotation was obtained using GOrilla [27]. Clustered network of the 200 upregulated proteins in spheroids treated with NaBut for 3 days E and F recovered spheroids (day 10). Clusters reflect connected portions of the network and correspond to functional categories of the proteins. Size of nodes represents p-value, color darkness represents the score (fold change enrichment times the p-value), and line thickness represents the score of interaction confidence retrieved from the software String (v11, https://string-db.org). The network was constructed by Cytoscape [20]