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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Investigation of reversible histone acetylation and dynamics in gene expression regulation using 3D liver spheroid model

Fig. 1

The proteome and metabolome of HepG2/C3A spheroids resemble a functional liver. A HepG2/C3A (human hepatocellular carcinoma) flat cells were cultivated in DMEM media supplemented with 10% FBS, until colonies reached 80% confluence. B Then, cells were trypsinized and plated on an ultra-low attachment 24-well plate containing microwells. C Spheroids were then detached from the plate, transferred to a bioreactor, and cultivated in the same media as flat cells (day 0). D Spheroids on day 5, E day 13, and F day 22. Images were acquired using PAULA Smart Cell Imager (Leica) with 10 × magnification. Scale bar = 100 µm. G Clustered network of the 200 upregulated proteins in HepG2/C3A spheroids. Clusters reflect connected portions of the network and correspond to functional categories of the proteins. The size of nodes represents p-value, color darkness represents the score (fold change enrichment times the p-value), and line thickness represents the score of interaction confidence retrieved from the software String (v11, https://string-db.org). The network was constructed by Cytoscape [20]. H Bar graphs show the daily secretion levels of the bile salts taurodeoxycholate, tauro α-muricholate, and taurochenodeoxycholate detected in the supernatant of spheroid culture

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