Fig. 4

Genomic distribution of cohesin and associated factors. a Heatmaps showing accumulation of the indicated proteins at CTCF and non-CTCF cohesin positions defined in MEFs in Fig. 1a. The latter were further separated in three clusters according to the presence of NIPBL and PDS5. Number of positions in each cluster is shown on the right. Datasets used in Additional file 2: Table S1. b Immunoblot analyses of immunoprecipitates obtained with anti-SMC1 from HeLa cell nuclear extracts. c The same volume of HeLa cell nuclear extract was immunodepleted from both PDS5 proteins or mock depleted as control (input). Both were next immunoprecipitated with anti-SMC1 and unbound and bound fractions analyzed by immunoblot. The ratio between the amount of NIPBL, PDS5, WAPL and cohesin (SMC1 or RAD21) pulled down from mock depleted and PDS5 depleted extracts was quantified in two independent experiments. d Distribution of cohesin positions for each cluster in the chromatin states described on the right. e. Heatmaps showing localization of the indicated proteins and histone marks around 24,388 TSSs. f Correlation between the presence of RNA polymerase II (Pol II) and NIPBL (top) or CTCF (bottom) at active TSSs