Fig. 1

STAG2 is the preferred variant at non-CTCF cohesin positions in MEFs. a Heatmaps showing ChIP-seq read distribution for cohesin and CTCF around non-CTCF (top cluster) and CTCF (bottom cluster) cohesin positions within a 5-kb window in MEFs. Data for STAG1 are from Busslinger et al. [32]. Signal intensity range at the bottom of each heatmap was selected to even cohesin signals at CTCF positions. b Read density plots showing SMC1, STAG1 and STAG2 distribution in the two types of positions. c Comparison of SMC1 distribution in WT and Stag1 KO MEFs (left) and WT and Stag2 KO MEFs (right). Same signal intensity range used for all heatmaps. d Boxplot representing average signal differences (log₂FC) between non-CTCF and CTCF cohesin positions when comparing Stag1 KO and Stag2 KO datasets with WT (see Methods). Student’s T test was used to assess statistical significance