Fig. 4
From: De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a
H3K23M differentially interacts with EHMT1/GLP and EHMT2/G9a in vitro and perturbs H3K9 and H3K23 methylation in vivo. A Recombinant EHMT1/GLP or EHMT2/G9a were incubated with H3K9me0, H3K9M, H3K23me0, or H3K23M peptides to probe for binding, in vitro, and binding was assessed by western blotting. B Histones from 50B11 cells expressing the indicated K-to-M mutations were analyzed using western blotting to determine the effect of these mutations on H3K9 and H3K23 methylation states in vivo