Fig. 2
From: De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a
Pharmacologic inhibition of EHMT1/GLP and EHMT2/GLP decreases H3K9 and H3K23 methylation in vitro. A A dose titration was performed using the competitive inhibitor UNC0642 with either EHMT1/GLP or EHMT2/G9a, and the enzyme activity in the presence of the inhibitor or DMSO (the vehicle) was measured using the MTase-Glo assay. B Recombinant histone H3.1 was incubated with either EHMT1/GLP or EHMT2/G9a, SAM, and either DMSO or 10 μM UNC0642 for 24 h, and subjected to western blotting to detect loss of various H3K9 and H3K23 methylation states in the in vitro HMT inhibition reactions