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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a

Fig. 1

EHMT1/GLP and EHMT2/G9a can de novo methylate histone H3K9 and H3K23 in vitro. A Recombinant histone H3.1 was incubated with SAM and either recombinant EHMT1/GLP or EHMT2/G9a for 24 h, and these in vitro HMT reactions were subjected to western blotting to detect the abundance of various H3K9 and H3K23 methylation states. B Mass spectrometry was used to validate the presence of specific methylation states catalyzed by EHMT1/GLP or EHMT2/G9a in the in vitro HMT assays. Representative spectra for H3K23me3 in the EHMT1/GLP HMT reactions and H3K23me2 in the EHMT2/G9a reactions are shown in panel B. Please also see Additional file 1: Fig. S1, which contains ions and mass error tables that correspond to this spectra, and contains spectra of additional methylation states detected in the in vitro HMT assays

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