Fig. 4
From: Sperm DNA methylation alterations from cannabis extract exposure are evident in offspring
Bisulfite Pyrosequencing of F1 tissues. Bisulfite pyrosequencing detected heritable changes at A Pxylp1 in F1 sperm; B Mtss1l in F1 hippocampus; and C Mtss1l in F1 NAc. CpG sites labeled with “*” correspond to those identified via WGBS in the LE v C or EE v C datasets. Black, control offspring; blue, EE offspring; green, LE offspring. Two-factor ANOVA (factor 1 = exposure status, factor 2 = CpG site) were significant for all genes. Significance indicators on graphs represent post hoc two-tailed t-tests. #p = 0.05, *p < 0.05, **p < 0.01