Fig. 2

Loss of a PDS5 protein does not cause mislocalization of the other PDS5 protein or the cohesin complex. A Western blot analysis of cohesin complex subunits in WT, Pds5a^−/−, and Pds5b^−/− mESC nuclear lysates. B Genome browser tracks of PDS5A ChIP-seq signal in WT and Pds5b^−/− mESCs and PDS5B signal in WT and Pds5a^−/− mESCs at the Sox17 gene locus. C MA plots showing differential enrichment of PDS5A signal between WT and Pds5b^−/− mESCs at conserved binding sites, as well as differential enrichment of PDS5B signal between WT and Pds5a^−/− mESCs at conserved binding sites. D Average signal plots of PDS5A ChIP-seq signal in WT and Pds5b^−/− mESCs, as well as PDS5B signal in WT and Pds5a^−/− mESCs, at CTCF sites, enhancers, and promoters. E Genome browser tracks of RAD21 ChIP-seq signal in WT, Pds5a^−/−, and Pds5b^−/− mESCs at the Klf4 gene locus. F MA plots showing differential enrichment of RAD21 signal between WT and Pds5a^−/− mESCs at conserved binding sites, as well between WT and Pds5b^−/− mESCs at conserved binding sites. G Average signal plots of RAD21 ChIP-seq signal in WT, Pds5a^−/−, and Pds5b^−/− mESCs at CTCF sites, enhancers, and promoters. H Genome browser tracks of RAD21 ChIP-seq signal in Pds5a^−/− siGLO and Pds5a^−/− siPds5b mESCs at the Sox2 gene locus. I Heatmaps of RAD21 ChIP-seq signal in Pds5a^−/− siGLO and Pds5a^−/− siPds5b mESCs at a union peak list of all RAD21 peaks in both conditions. J Average signal plots of RAD21 ChIP-seq signal in Pds5a^−/− siGLO and Pds5a^−/− siPds5b mESCs at CTCF sites, enhancers, and promoters. K MA plot showing differential RAD21 ChIP-seq signal between Pds5a^−/− siPds5b and Pds5a^−/− siGLO mESCs at conserved binding sites