Fig. 4

Parental origin-specific binding of MSH2 and MSH6 to the ICRs. a Box plot showing the methylation level of non-repetitive MSH2-bound regions (left) and MSH2-bound ICRs (right). b Electropherograms showing the DNA methylation level of the Inpp5f and Gnas ICRs in genomic DNA, ChIP DNA obtained with anti-H3K9me3, anti-H3K4me3 antibodies and Bio-ChIP DNA pulled down with streptavidin in Msh2-AviTag-transfected ESCs, and revealed by bisulfite sequencing. Methylation levels were determined from the ratio between methylated (C) and unmethylated (T) cytosines. c Electropherograms showing the allele-specific binding of MSH6 in hybrid WT and Zfp57-/- ESCs at the Plagl1 and Inpp5f ICRs. The SNVs associated with the maternal JF1 or paternal Black/6 (B6) alleles are shown at the top. The relative enrichment of the maternal over the paternal allele is reported below the electropherogram. Black arrows indicate the SNVs. d Bar plot showing relative enrichment of Biotin-tagged MSH2 protein to ICRs in WT and Zfp57-/- ESCs. ChIP values were expressed as % input and normalized to the Smg7 region that is used as a positive control