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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Multi-omics analyses of MEN1 missense mutations identify disruption of menin–MLL and menin–JunD interactions as critical requirements for molecular pathogenicity

Fig. 4

Quantitative proteomics of cytoplasmic GFP-tagged WT menin or mutant menin expressed in HeLa cells. A Volcano and stoichiometry plots for the significant interactors of WT menin-GFP from the cytoplasmic fraction. Control and GFP stand for pulldown with control agarose beads versus GFP-Trap agarose beads, respectively. Stringent criteria (FDR = 0.01, s0 = 2) were applied for significance scores. B iBAQ-based stoichiometries of the top 10 cytoplasmic interactors normalized to the WT or mutant menin-GFP bait protein. Results shown represent Intensity Based Absolute Quantification with whiskers indicating standard deviations. Please note that the MaxQuant search identified RPS27A and UBA52 as cytoplasmic menin inhibitors, but manual inspection of peptides assigned to RPS27A and UBA52 uniquely map to the ubiquitin part of these ubiquitin fusion proteins and no RPS27A nor UBA52 specific peptides could be identified

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Epigenetics & Chromatin

ISSN: 1756-8935