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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Multi-omics analyses of MEN1 missense mutations identify disruption of menin–MLL and menin–JunD interactions as critical requirements for molecular pathogenicity

Fig. 3

Quantitative proteomics of nuclear GFP-tagged WT menin or mutant menin expressed in HeLa cells. A Volcano and stoichiometry plots of significant interactors of WT menin-GFP isolated from nuclear extracts. Control and GFP stand for pulldown with control agarose beads versus GFP-Trap agarose beads, respectively. Stringent criteria (FDR = 0.01, s0 = 2) were applied for significance scores. B Stoichiometry plots of all mutant menin-GFP proteins for subunits of MLL1/MLL2 (red bars)- and JunD (blue bars)-containing complexes. All interactors were normalized to the menin-GFP bait. C Summary of MLL1/MLL2 complex (red bars) and of JunD complex (blue bars) interaction stoichiometries of menin mutants relative to WT menin-GFP. Results shown represent iBAQ (intensity Based Absolute Quantification) values with whiskers indicating standard deviations

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