Fig. 2From: Multi-omics analyses of MEN1 missense mutations identify disruption of menin–MLL and menin–JunD interactions as critical requirements for molecular pathogenicityExpression levels of menin proteins after transient transfections of HEK293 cells and or stable integration in stable HeLa cell lines. A Protein expression was assessed by ectopic expression of MEN1 WT and mutant cDNA in HEK293T cells and subsequent immunoblotting using menin antibodies or α-tubulin antibodies as a loading control. B Immunoblots upon doxycycline induction of stable HeLa cell lines showing expression of GFP-tagged menin using menin antibodies or α-tubulin antibodies as a loading control. C qPCR analysis of MEN1 RNA levels. C Fluorescence microscopy images of GFP-tagged WT menin and mutant proteins stained with anti-GFP antibody and counter-stained with DAPIBack to article page