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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Multi-omics analyses of MEN1 missense mutations identify disruption of menin–MLL and menin–JunD interactions as critical requirements for molecular pathogenicity

Fig. 2

Expression levels of menin proteins after transient transfections of HEK293 cells and or stable integration in stable HeLa cell lines. A Protein expression was assessed by ectopic expression of MEN1 WT and mutant cDNA in HEK293T cells and subsequent immunoblotting using menin antibodies or α-tubulin antibodies as a loading control. B Immunoblots upon doxycycline induction of stable HeLa cell lines showing expression of GFP-tagged menin using menin antibodies or α-tubulin antibodies as a loading control. C qPCR analysis of MEN1 RNA levels. C Fluorescence microscopy images of GFP-tagged WT menin and mutant proteins stained with anti-GFP antibody and counter-stained with DAPI

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