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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Open chromatin analysis in Trypanosoma cruzi life forms highlights critical differences in genomic compartments and developmental regulation at tDNA loci

Fig. 6

FAIRE enrichment at tDNA loci. A IGV snapshot of a representative tDNA cluster (black box) showing FAIRE enrichment in epimastigote (red) tracks over metacyclic trypomastigotes (blue). B Boxplot of RPGC-normalized tag counts in tDNA features (*** Wilcoxon–Mann–Whitney test with p-value = 0.001). C Hierarchical cluster analysis of FAIRE-seq data at tDNA loci (reciprocal ratio). The number of tDNAs in each cluster is depicted below. D Distribution of tDNAs from each cluster of C according to their location relative to the adjacent PTU transcription direction. E RNA-FISH analysis of Asp-GUC 3’ tRNAs in epimastigotes and metacyclics. F Northern blot assays using 20 pmol of biotin- labeled probes for Asp, Glu tRNAs, and 5S RNA. Total RNA were fractionated in 15% polyacrylamide gels and transferred to nylon membranes

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Epigenetics & Chromatin

ISSN: 1756-8935